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Image Search Results
Journal: Cell reports
Article Title: Complement C4A Regulates Autoreactive B Cells in Murine Lupus
doi: 10.1016/j.celrep.2020.108330
Figure Lengend Snippet: (A) Splenic GC B cell population (B220 + CD38 dim/− GL7 high ) analyzed by flow cytometry in 15 wo C4A, C4B, and C4 +/+ 564Igi. Means ± SEMs; 1-way ANOVA with Tukey’s test; 1 dot represents 1 mouse; n > 6. (B–D) Analysis of GC structures by immunohistochemistry. (B) Representative images of spleen follicles. Marginal zone macrophages: CD169 (red), B cell zone: B220 (blue), T cell zone: CD3 (green) and GC: GL7 (white); the scale bar represents 100 μm. (C) Frequency of GL7 + follicles. Means ± SEMs; 1-way ANOVA with Tukey’s test; 1 dot represents 1 mouse; n > 8 for each 564Igi strain. (D) Quantification of individual GL7 + area using ImageJ software. Means ± SEMs; 1-way ANOVA with Tukey’s test; 1 dot represents 1 GL7 + area; n > 7 animals for each 564Igi strain. (E) Serum anti-nucleoli IgG titers measured by ELISA. Means ± SEMs; 1 dot represents 1 mouse; n > 8. *p < 0.05, **p < 0.01, ****p < 0.0001). See also .
Article Snippet:
Techniques: Flow Cytometry, Immunohistochemistry, Software, Enzyme-linked Immunosorbent Assay
Journal: Cell reports
Article Title: Complement C4A Regulates Autoreactive B Cells in Murine Lupus
doi: 10.1016/j.celrep.2020.108330
Figure Lengend Snippet: (A) Volcano plot comparison of median IgG signal intensities in C4A 564Igi (n = 8) relative to C4B 564Igi (n = 10) mice. Log2(fold change) appears on the x axis. Significance (presented as −10 × log10[p value]) is on the y axis. The p value was obtained running multiple unpaired t tests corrected with the Holm-Sidak method. One dot represents 1 self-Ag. (B–D) Dosage of anti-Ro60, -BPI, and -CTGF IgG titers in serum of 15–20 wo C4 −/− , C4A, C4B, and C4 +/+ 564Igi by ELISA. Means ± SEMs; 1 dot represents 1 mouse; n > 4; 1-way ANOVA with Tukey’s test; *p < 0.05. See also and .
Article Snippet:
Techniques: Comparison, Enzyme-linked Immunosorbent Assay
Journal: Cell reports
Article Title: Complement C4A Regulates Autoreactive B Cells in Murine Lupus
doi: 10.1016/j.celrep.2020.108330
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Purification, Recombinant, Software
Journal: Immunity
Article Title: LAG-3 Inhibitory Receptor Expression Identifies Immunosuppressive Natural Regulatory Plasma Cells
doi: 10.1016/j.immuni.2018.06.007
Figure Lengend Snippet:
Article Snippet:
Techniques: Cell Isolation, Recombinant, Electron Microscopy, Staining, One Step RT-PCR, Reverse Transcription, SYBR Green Assay, DNA Methylation Assay, Expressing, Liposomes, Labeling, Sample Prep, Methylation, Purification, Mutagenesis, Plasmid Preparation, Transfection